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In vitro reconstitution assays of microtubule amplification and lattice repair by the microtubule severing enzymes katanin and spastin

Microtubules are non-covalent dynamic polymers essential for the life of all eukaryotic cells. Their dynamic behavior is regulated by a large array of cellular effectors. In vitro microtubule assays have been instrumental in dissecting the mechanism of microtubule associated proteins. In this chapte...

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Publicat a:Methods Mol Biol
Autors principals: Vemu, Annapurna, Szczesna, Ewa, Roll-Mecak, Antonina
Format: Artigo
Idioma:Inglês
Publicat: 2020
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7153568/
https://ncbi.nlm.nih.gov/pubmed/31879896
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-0716-0219-5_3
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