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Continuous evolution of SpCas9 variants compatible with non-G PAMs

The targeting scope of Streptococcus pyogenes Cas9 (SpCas9) and its engineered variants is largely restricted to protospacer-adjacent motif (PAM) sequences containing Gs. Here, we report the evolution of three new SpCas9 variants that collectively recognize NRNH PAMs (where R = A or G and H = A, C,...

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Publicat a:Nat Biotechnol
Autors principals: Miller, Shannon M., Wang, Tina, Randolph, Peyton B., Arbab, Mandana, Shen, Max W., Huang, Tony P., Matuszek, Zaneta, Newby, Gregory A., Rees, Holly A., Liu, David R.
Format: Artigo
Idioma:Inglês
Publicat: 2020
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7145744/
https://ncbi.nlm.nih.gov/pubmed/32042170
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41587-020-0412-8
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