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Minimal PAM specificity of a highly similar SpCas9 ortholog
RNA-guided DNA endonucleases of the CRISPR-Cas system are widely used for genome engineering and thus have numerous applications in a wide variety of fields. CRISPR endonucleases, however, require a specific protospacer adjacent motif (PAM) flanking the target site, thus constraining their targetabl...
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| Опубликовано в: : | Sci Adv |
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| Главные авторы: | , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
American Association for the Advancement of Science
2018
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6200363/ https://ncbi.nlm.nih.gov/pubmed/30397647 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/sciadv.aau0766 |
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