Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

A key limitation to the use of CRISPR-Cas9 proteins for genome editing and other applications is the requirement that a protospacer adjacent motif (PAM) be present at the target site. For the most commonly used Cas9 from Streptococcus pyogenes (SpCas9), this PAM requirement is NGG. No natural or eng...

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Udgivet i:Nature
Main Authors: Hu, Johnny H., Miller, Shannon M., Geurts, Maarten H., Tang, Weixin, Chen, Liwei, Sun, Ning, Zeina, Christina M., Gao, Xue, Rees, Holly A., Lin, Zhi, Liu, David R.
Format: Artigo
Sprog:Inglês
Udgivet: 2018
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5951633/
https://ncbi.nlm.nih.gov/pubmed/29512652
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nature26155
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