Engineered AsCas12a Variants with Enhanced Activity and Broadened PAM Compatibility

CRISPR-Cas12a (Cpf1) is a type V CRISPR effector RNA-guided DNA endonuclease with utility in synthetic biology and genome engineering. As an alternative to the commonly used Streptococcus pyogenes Cas9 (SpCas9), Cas12a recognizes TTTV (V = A/G/C) PAM sequences, which permits editing in AT-rich regio...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:J Biomol Tech
Prif Awduron: Collingwood, Michael A., Vakulskas, Christopher A., Zhang, Liyang, Turk, Rolf, Bode, Nicole M., Jacobi, Ashley M., McNeill, Matthew S., McQuisten, Kyle, Thommandru, Bernice, Behlke, Mark A.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Association of Biomolecular Resource Facilities 2019
Pynciau:
Scientific Session Abstracts
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC6938062/
Tagiau: Ychwanegu Tag
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