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The post-PAM interaction of RNA-guided spCas9 with DNA dictates its target binding and dissociation

Cas9 is an RNA-guided endonuclease that targets complementary DNA for cleavage and has been repurposed for many biological usages. Cas9 activities are governed by its direct interactions with DNA. However, information about this interplay and the mechanism involved in its direction of Cas9 activity...

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Vydáno v:Sci Adv
Hlavní autoři: Zhang, Qian, Wen, Fengcai, Zhang, Siqi, Jin, Jiachuan, Bi, Lulu, Lu, Ying, Li, Ming, Xi, Xu-Guang, Huang, Xingxu, Shen, Bin, Sun, Bo
Médium: Artigo
Jazyk:Inglês
Vydáno: American Association for the Advancement of Science 2019
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6853773/
https://ncbi.nlm.nih.gov/pubmed/31763447
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/sciadv.aaw9807
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