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The post-PAM interaction of RNA-guided spCas9 with DNA dictates its target binding and dissociation

Cas9 is an RNA-guided endonuclease that targets complementary DNA for cleavage and has been repurposed for many biological usages. Cas9 activities are governed by its direct interactions with DNA. However, information about this interplay and the mechanism involved in its direction of Cas9 activity...

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Detalhes bibliográficos
Publicado no:Sci Adv
Main Authors: Zhang, Qian, Wen, Fengcai, Zhang, Siqi, Jin, Jiachuan, Bi, Lulu, Lu, Ying, Li, Ming, Xi, Xu-Guang, Huang, Xingxu, Shen, Bin, Sun, Bo
Formato: Artigo
Idioma:Inglês
Publicado em: American Association for the Advancement of Science 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6853773/
https://ncbi.nlm.nih.gov/pubmed/31763447
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/sciadv.aaw9807
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