The post-PAM interaction of RNA-guided spCas9 with DNA dictates its target binding and dissociation

Cas9 is an RNA-guided endonuclease that targets complementary DNA for cleavage and has been repurposed for many biological usages. Cas9 activities are governed by its direct interactions with DNA. However, information about this interplay and the mechanism involved in its direction of Cas9 activity...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Sci Adv
Prif Awduron: Zhang, Qian, Wen, Fengcai, Zhang, Siqi, Jin, Jiachuan, Bi, Lulu, Lu, Ying, Li, Ming, Xi, Xu-Guang, Huang, Xingxu, Shen, Bin, Sun, Bo
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: American Association for the Advancement of Science 2019
Pynciau:
Research Articles
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC6853773/
https://ncbi.nlm.nih.gov/pubmed/31763447
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/sciadv.aaw9807
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