Measuring mRNA translation in neuronal processes and somata by tRNA-FRET

In neurons, the specific spatial and temporal localization of protein synthesis is of great importance for function and survival. Here, we visualized tRNA and protein synthesis events in fixed and live mouse primary cortical culture using fluorescently-labeled tRNAs. We were able to characterize the...

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Bibliographic Details
Published in:Nucleic Acids Res
Main Authors: Koltun, Bella, Ironi, Sivan, Gershoni-Emek, Noga, Barrera, Iliana, Hleihil, Mohammad, Nanguneri, Siddharth, Sasmal, Ranjan, Agasti, Sarit S, Nair, Deepak, Rosenblum, Kobi
Format: Artigo
Language:Inglês
Published: Oxford University Press 2020
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Methods Online
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7102941/
https://ncbi.nlm.nih.gov/pubmed/31974573
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa042
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