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Measuring mRNA translation in neuronal processes and somata by tRNA-FRET

In neurons, the specific spatial and temporal localization of protein synthesis is of great importance for function and survival. Here, we visualized tRNA and protein synthesis events in fixed and live mouse primary cortical culture using fluorescently-labeled tRNAs. We were able to characterize the...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Koltun, Bella, Ironi, Sivan, Gershoni-Emek, Noga, Barrera, Iliana, Hleihil, Mohammad, Nanguneri, Siddharth, Sasmal, Ranjan, Agasti, Sarit S, Nair, Deepak, Rosenblum, Kobi
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7102941/
https://ncbi.nlm.nih.gov/pubmed/31974573
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa042
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