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Monitoring Protein Synthesis in Living Cells with Fluorescent Labeled tRNA FRET Pairs

We introduce Protein Synthesis Monitoring (PSM) – a technique to monitor protein synthesis in live cells. In PSM, we transfect cells with tRNA labeled as FRET donors and acceptors. A FRET signal is generated only when a donor- and an acceptor-labeled tRNA come in close contact (< 7nM), as they do...

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Bibliografske podrobnosti
Main Authors: Barhoom, Sima, Farrel, Ian, Dahary, Dvir, Ehrlich, Marcelo, Cooperman, Barry S., Elroy-Stein, Orna, Smilansky, Zeev
Format: Artigo
Jezik:Inglês
Izdano: Association of Biomolecular Resource Facilities 2013
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Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3635411/
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