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Quantitative single cell monitoring of protein synthesis at subcellular resolution using fluorescently labeled tRNA
We have developed a novel technique of using fluorescent tRNA for translation monitoring (FtTM). FtTM enables the identification and monitoring of active protein synthesis sites within live cells at submicron resolution through quantitative microscopy of transfected bulk uncharged tRNA, fluorescentl...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3201886/ https://ncbi.nlm.nih.gov/pubmed/21795382 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr601 |
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