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Quantitative single cell monitoring of protein synthesis at subcellular resolution using fluorescently labeled tRNA

We have developed a novel technique of using fluorescent tRNA for translation monitoring (FtTM). FtTM enables the identification and monitoring of active protein synthesis sites within live cells at submicron resolution through quantitative microscopy of transfected bulk uncharged tRNA, fluorescentl...

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Bibliografiske detaljer
Main Authors: Barhoom, Sima, Kaur, Jaskiran, Cooperman, Barry S., Smorodinsky, Nechama I., Smilansky, Zeev, Ehrlich, Marcelo, Elroy-Stein, Orna
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2011
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3201886/
https://ncbi.nlm.nih.gov/pubmed/21795382
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr601
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