Measuring mRNA translation in neuronal processes and somata by tRNA-FRET

In neurons, the specific spatial and temporal localization of protein synthesis is of great importance for function and survival. Here, we visualized tRNA and protein synthesis events in fixed and live mouse primary cortical culture using fluorescently-labeled tRNAs. We were able to characterize the...

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Dettagli Bibliografici
Pubblicato in:Nucleic Acids Res
Autori principali: Koltun, Bella, Ironi, Sivan, Gershoni-Emek, Noga, Barrera, Iliana, Hleihil, Mohammad, Nanguneri, Siddharth, Sasmal, Ranjan, Agasti, Sarit S, Nair, Deepak, Rosenblum, Kobi
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2020
Soggetti:
Methods Online
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7102941/
https://ncbi.nlm.nih.gov/pubmed/31974573
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa042
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