Measuring mRNA translation in neuronal processes and somata by tRNA-FRET

In neurons, the specific spatial and temporal localization of protein synthesis is of great importance for function and survival. Here, we visualized tRNA and protein synthesis events in fixed and live mouse primary cortical culture using fluorescently-labeled tRNAs. We were able to characterize the...

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Dades bibliogràfiques
Publicat a:Nucleic Acids Res
Autors principals: Koltun, Bella, Ironi, Sivan, Gershoni-Emek, Noga, Barrera, Iliana, Hleihil, Mohammad, Nanguneri, Siddharth, Sasmal, Ranjan, Agasti, Sarit S, Nair, Deepak, Rosenblum, Kobi
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2020
Matèries:
Methods Online
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7102941/
https://ncbi.nlm.nih.gov/pubmed/31974573
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa042
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