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Function-based high-throughput screening for antibody antagonists and agonists against G protein-coupled receptors

Hybridoma and phage display are two powerful technologies for isolating target-specific monoclonal antibodies based on the binding. However, for complex membrane proteins, such as G protein-coupled receptors (GPCRs), binding-based screening rarely results in functional antibodies. Here we describe a...

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Bibliografische gegevens
Gepubliceerd in:Commun Biol
Hoofdauteurs: Ren, Huanhuan, Li, Jian, Zhang, Ning, Hu, Liaoyuan A., Ma, Yingli, Tagari, Philip, Xu, Jianqing, Zhang, Mei-Yun
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Nature Publishing Group UK 2020
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7099005/
https://ncbi.nlm.nih.gov/pubmed/32218528
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s42003-020-0867-7
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