Efficient RNP-directed Human Gene Targeting Reveals SPDEF Is Required for IL-13–induced Mucostasis

Primary human bronchial epithelial cell (HBEC) cultures are a useful model for studies of lung health and major airway diseases. However, mechanistic studies have been limited by our ability to selectively disrupt specific genes in these cells. Here we optimize methods for gene targeting in HBECs by...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Am J Respir Cell Mol Biol
Prif Awduron: Koh, Kyung Duk, Siddiqui, Sana, Cheng, Dan, Bonser, Luke R., Sun, Dingyuan I., Zlock, Lorna T., Finkbeiner, Walter E., Woodruff, Prescott G., Erle, David J.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: American Thoracic Society 2020
Pynciau:
Original Research
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC7055692/
https://ncbi.nlm.nih.gov/pubmed/31596609
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1165/rcmb.2019-0266OC
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