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Efficient RNP-directed Human Gene Targeting Reveals SPDEF Is Required for IL-13–induced Mucostasis

Primary human bronchial epithelial cell (HBEC) cultures are a useful model for studies of lung health and major airway diseases. However, mechanistic studies have been limited by our ability to selectively disrupt specific genes in these cells. Here we optimize methods for gene targeting in HBECs by...

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Detalhes bibliográficos
Publicado no:Am J Respir Cell Mol Biol
Main Authors: Koh, Kyung Duk, Siddiqui, Sana, Cheng, Dan, Bonser, Luke R., Sun, Dingyuan I., Zlock, Lorna T., Finkbeiner, Walter E., Woodruff, Prescott G., Erle, David J.
Formato: Artigo
Idioma:Inglês
Publicado em: American Thoracic Society 2020
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7055692/
https://ncbi.nlm.nih.gov/pubmed/31596609
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1165/rcmb.2019-0266OC
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