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A Click Chemistry Approach Reveals the Chromatin-Dependent Histone H3K36 Deacylase Nature of SIRT7
Using an engineered pyrrolysyl-tRNA synthetase mutant together with [Formula: see text] , we have genetically encoded N(ε)-(7-azidoheptanoyl)-L-lysine (AzHeK) by amber codon in Escherichia coli for recombinant expression of a number of AzHeK-containing histone H3 proteins. We assembled in vitro acyl...
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| Pubblicato in: | J Am Chem Soc |
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| Autori principali: | , , , , , , , , , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2019
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6812484/ https://ncbi.nlm.nih.gov/pubmed/30653310 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.8b12083 |
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