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A Click Chemistry Approach Reveals the Chromatin-Dependent Histone H3K36 Deacylase Nature of SIRT7

Using an engineered pyrrolysyl-tRNA synthetase mutant together with [Formula: see text] , we have genetically encoded N(ε)-(7-azidoheptanoyl)-L-lysine (AzHeK) by amber codon in Escherichia coli for recombinant expression of a number of AzHeK-containing histone H3 proteins. We assembled in vitro acyl...

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Bibliografski detalji
Izdano u:J Am Chem Soc
Glavni autori: Wang, Wesley Wei, Angulo-Ibanez, Maria, Lyu, Jie, Kurra, Yadagiri, Tong, Zhen, Wu, Bo, Zhang, Ling, Sharma, Vangmayee, Zhou, Jennifer, Lin, Hening, Gao, Yi Qin, Li, Wei, Chua, Katrin F., Liu, Wenshe Ray
Format: Artigo
Jezik:Inglês
Izdano: 2019
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6812484/
https://ncbi.nlm.nih.gov/pubmed/30653310
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.8b12083
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