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Evolving the N-terminal Domain of Pyrrolysyl-tRNA Synthetase for Improved Incorporation of Noncanonical Amino Acids
By evolving the N-terminal domain of Methanosarcina mazei pyrrolysyl-tRNA synthetase (PylRS) that directly interacts with tRNA(Pyl), a mutant clone that displays improved amber suppression efficiency for the genetic incorporation of N(ε)-(tert-butoxycarbonyl)-L-lysine three-fold more than the wild t...
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| Publicado no: | Chembiochem |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5989136/ https://ncbi.nlm.nih.gov/pubmed/29096043 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201700268 |
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