Caricamento...

Measurement of Kinetic Isotope Effects in an Enzyme-Catalyzed Reaction by Continuous-Flow Mass Spectrometry

Kinetic isotope effects (KIEs) provide powerful probes of the mechanisms of enzyme-catalyzed reactions. In this chapter, we describe the use of continuous-flow mass spectrometry to determine the deuterium KIE for the enzyme N-acetylpolyamine oxidase based on the ratio of labeled and unlabeled produc...

Descrizione completa

Salvato in:
Dettagli Bibliografici
Pubblicato in:Methods Enzymol
Autori principali: Roberts, Kenneth M., Fitzpatrick, Paul F.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2017
Soggetti:
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6767924/
https://ncbi.nlm.nih.gov/pubmed/28911769
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2017.07.001
Tags: Aggiungi Tag
Nessun Tag, puoi essere il primo ad aggiungerne! !