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Measurement of Kinetic Isotope Effects in an Enzyme-Catalyzed Reaction by Continuous-Flow Mass Spectrometry

Kinetic isotope effects (KIEs) provide powerful probes of the mechanisms of enzyme-catalyzed reactions. In this chapter, we describe the use of continuous-flow mass spectrometry to determine the deuterium KIE for the enzyme N-acetylpolyamine oxidase based on the ratio of labeled and unlabeled produc...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:Methods Enzymol
Egile Nagusiak: Roberts, Kenneth M., Fitzpatrick, Paul F.
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 2017
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC6767924/
https://ncbi.nlm.nih.gov/pubmed/28911769
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2017.07.001
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