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Inverse Solvent Isotope Effects in Enzyme-Catalyzed Reactions
Solvent isotope effects have long been used as a mechanistic tool for determining enzyme mechanisms. Most commonly, macroscopic rate constants such as k(cat) and k(cat)/K(m) are found to decrease when the reaction is performed in D(2)O for a variety of reasons including the transfer of protons. Unde...
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| Publicado en: | Molecules |
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| Autores principales: | , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
MDPI
2020
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7221790/ https://ncbi.nlm.nih.gov/pubmed/32326332 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/molecules25081933 |
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