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Inverse Solvent Isotope Effects in Enzyme-Catalyzed Reactions

Solvent isotope effects have long been used as a mechanistic tool for determining enzyme mechanisms. Most commonly, macroscopic rate constants such as k(cat) and k(cat)/K(m) are found to decrease when the reaction is performed in D(2)O for a variety of reasons including the transfer of protons. Unde...

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Detalhes bibliográficos
Publicado no:Molecules
Main Authors: Fernandez, Patrick L., Murkin, Andrew S.
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7221790/
https://ncbi.nlm.nih.gov/pubmed/32326332
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/molecules25081933
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