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Guidelines for SHAPE reagent choice and detection strategy for RNA structure probing studies

Chemical probing is an important tool for characterizing the complex folded structures of RNA molecules, many of which play key cellular roles. Electrophilic SHAPE reagents create adducts at the 2'-hydroxyl position on the RNA backbone of flexible ribonucleotides with relatively little dependen...

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Bibliografische gegevens
Gepubliceerd in:Biochemistry
Hoofdauteurs: Busan, Steven, Weidmann, Chase A., Sengupta, Arnab, Weeks, Kevin M.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2019
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6712974/
https://ncbi.nlm.nih.gov/pubmed/31117385
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.8b01218
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