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Accurate detection of chemical modifications in RNA by mutational profiling (MaP) with ShapeMapper 2

Mutational profiling (MaP) enables detection of sites of chemical modification in RNA as sequence changes during reverse transcription (RT), subsequently read out by massively parallel sequencing. We introduce ShapeMapper 2, which integrates careful handling of all classes of adduct-induced sequence...

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Detalhes bibliográficos
Publicado no:RNA
Main Authors: Busan, Steven, Weeks, Kevin M.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5769742/
https://ncbi.nlm.nih.gov/pubmed/29114018
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.061945.117
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