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Bridge helix of Cas9 modulates target DNA cleavage and mismatch tolerance

CRISPR-Cas systems are RNA-guided nucleases that provide adaptive immune protection for bacteria and archaea against intruding genomic materials. The programmable nature of CRISPR targeting mechanisms has enabled their adaptation as powerful genome engineering tools. Cas9, a type II CRISPR effector...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Biochemistry
Hauptverfasser: Babu, Kesavan, Amrani, Nadia, Jiang, Wei, Yogesha, S.D., Nguyen, Richard, Qin, Peter Z., Rajan, Rakhi
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2019
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6496953/
https://ncbi.nlm.nih.gov/pubmed/30916546
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.8b01241
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