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Distinct patterns of Cas9 mismatch tolerance in vitro and in vivo

Cas9, a CRISPR-associated RNA-guided nuclease, has been rapidly adopted as a tool for biochemical and genetic manipulation of DNA. Although complexes between Cas9 and guide RNAs (gRNAs) offer remarkable specificity and versatility for genome manipulation, mis-targeted events occur. To extend the und...

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Bibliografiska uppgifter
I publikationen:Nucleic Acids Res
Huvudupphovsmän: Fu, Becky X.H., St. Onge, Robert P., Fire, Andrew Z., Smith, Justin D.
Materialtyp: Artigo
Språk:Inglês
Publicerad: Oxford University Press 2016
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC4914125/
https://ncbi.nlm.nih.gov/pubmed/27198218
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkw417
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