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Generation of single-copy transgenic mouse embryos directly from ES cells by tetraploid embryo complementation

BACKGROUND: Transgenic mice have been used extensively to analyze gene function. Unfortunately, traditional transgenic procedures have only limited use in analyzing alleles that cause lethality because lines of founder mice cannot be established. This is frustrating given that such alleles often rev...

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Udgivet i:BMC Biotechnol
Principais autores: Misra, Ravi P, Bronson, Sarah K, Xiao, Qi, Garrison, Wendy, Li, Jixuan, Zhao, Roong, Duncan, Stephen A
Format: Artigo
Sprog:Inglês
Udgivet: BMC 2001
Fag:
Methodology Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC64498/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11782291/
https://ncbi.nlm.nih.govhttps://doi.org/10.1186/1472-6750-1-12
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