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Targeted Single Primer Enrichment Sequencing with Single End Duplex-UMI

For specific detection of somatic variants at very low levels, artifacts from the NGS workflow have to be eliminated. Various approaches using unique molecular identifiers (UMI) to analytically remove NGS artifacts have been described. Among them, Duplex-seq was shown to be highly effective, by leve...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Peng, Quan, Xu, Chang, Kim, Daniel, Lewis, Marcus, DiCarlo, John, Wang, Yexun
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6423013/
https://ncbi.nlm.nih.gov/pubmed/30886209
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-41215-z
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