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Targeted Single Primer Enrichment Sequencing with Single End Duplex-UMI

For specific detection of somatic variants at very low levels, artifacts from the NGS workflow have to be eliminated. Various approaches using unique molecular identifiers (UMI) to analytically remove NGS artifacts have been described. Among them, Duplex-seq was shown to be highly effective, by leve...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Sci Rep
Hauptverfasser: Peng, Quan, Xu, Chang, Kim, Daniel, Lewis, Marcus, DiCarlo, John, Wang, Yexun
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group UK 2019
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6423013/
https://ncbi.nlm.nih.gov/pubmed/30886209
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-41215-z
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