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Targeted Single Primer Enrichment Sequencing with Single End Duplex-UMI

For specific detection of somatic variants at very low levels, artifacts from the NGS workflow have to be eliminated. Various approaches using unique molecular identifiers (UMI) to analytically remove NGS artifacts have been described. Among them, Duplex-seq was shown to be highly effective, by leve...

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Bibliografiske detaljer
Udgivet i:Sci Rep
Main Authors: Peng, Quan, Xu, Chang, Kim, Daniel, Lewis, Marcus, DiCarlo, John, Wang, Yexun
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2019
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6423013/
https://ncbi.nlm.nih.gov/pubmed/30886209
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-41215-z
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