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Targeted Single Primer Enrichment Sequencing with Single End Duplex-UMI

For specific detection of somatic variants at very low levels, artifacts from the NGS workflow have to be eliminated. Various approaches using unique molecular identifiers (UMI) to analytically remove NGS artifacts have been described. Among them, Duplex-seq was shown to be highly effective, by leve...

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Detalles Bibliográficos
Publicado en:Sci Rep
Main Authors: Peng, Quan, Xu, Chang, Kim, Daniel, Lewis, Marcus, DiCarlo, John, Wang, Yexun
Formato: Artigo
Idioma:Inglês
Publicado: Nature Publishing Group UK 2019
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC6423013/
https://ncbi.nlm.nih.gov/pubmed/30886209
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-41215-z
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