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CRISPR-Cas9 circular permutants as programmable scaffolds for genome modification

The ability to engineer natural proteins is pivotal to a future, pragmatic biology. CRISPR proteins have revolutionized genome modification, yet the CRISPR-Cas9 scaffold is not ideal for fusions or activation by cellular triggers. Here we show that topological rearrangement of Cas9 using circular pe...

Täydet tiedot

Tallennettuna:
Bibliografiset tiedot
Julkaisussa:Cell
Päätekijät: Oakes, Benjamin L., Fellmann, Christof, Rishi, Harneet, Taylor, Kian L., Ren, Shawn M., Nadler, Dana C., Yokoo, Rayka, Arkin, Adam P., Doudna, Jennifer A., Savage, David F.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 2019
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC6414052/
https://ncbi.nlm.nih.gov/pubmed/30633905
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.cell.2018.11.052
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