Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch

The CRISPR-associated protein Cas9 from Streptococcus pyogenes is an RNA-guided DNA endonuclease with widespread utility for genome modification. However, the structural constraints limiting the engineering of Cas9 have not been determined. Here we experimentally profile Cas9 using randomized insert...

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Bibliographic Details
Published in:Nat Biotechnol
Main Authors: Oakes, Benjamin L, Nadler, Dana C., Flamholz, Avi, Fellmann, Christof, Staahl, Brett T., Doudna, Jennifer A., Savage, David F.
Format: Artigo
Language:Inglês
Published: 2016
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4900928/
https://ncbi.nlm.nih.gov/pubmed/27136077
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.3528
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