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Protein engineering of Cas9 for enhanced function
CRISPR/Cas systems act to protect the cell from invading nucleic acids in many bacteria and archaea. The bacterial immune protein Cas9 is a component of one of these CRISPR/Cas systems and has recently been adapted as a tool for genome editing. Cas9 is easily targeted to bind and cleave a DNA sequen...
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| Publicado no: | Methods Enzymol |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4641071/ https://ncbi.nlm.nih.gov/pubmed/25398355 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/B978-0-12-801185-0.00024-6 |
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