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Programmable RNA recognition and cleavage by CRISPR/Cas9
The CRISPR-associated protein Cas9 is an RNA-guided DNA endonuclease that uses RNA:DNA complementarity to identify target sites for sequence-specific doublestranded DNA (dsDNA) cleavage(1-5). In its native context, Cas9 acts on DNA substrates exclusively because both binding and catalysis require re...
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| Izdano u: | Nature |
|---|---|
| Glavni autori: | , , , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2014
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4268322/ https://ncbi.nlm.nih.gov/pubmed/25274302 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nature13769 |
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