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RNA-binding and HEPN-nuclease activation are decoupled in CRISPR-Cas13a

CRISPR-Cas13a enzymes are RNA-guided, RNA-activated ribonucleases. Their properties have been exploited as powerful tools for RNA detection, RNA imaging and RNA regulation. However, the relationship between target RNA binding and HEPN (higher-eukaryotes-and-prokaryotes nucleotide-binding)- domain nu...

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Détails bibliographiques
Publié dans:Cell Rep
Auteurs principaux: Tambe, Akshay, East-Seletsky, Alexandra, Knott, Gavin J., Doudna, Jennifer A., O’Connell, Mitchell R.
Format: Artigo
Langue:Inglês
Publié: 2018
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6085867/
https://ncbi.nlm.nih.gov/pubmed/30044970
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.celrep.2018.06.105
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