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Conformational control of DNA target cleavage by CRISPR–Cas9

Cas9 is an RNA-guided DNA endonuclease that targets foreign DNA for destruction as part of a bacterial adaptive immune system mediated by CRISPR (clustered regularly interspaced short palindromic repeats)(1,2). Together with single-guide RNAs (sgRNA)(3), Cas9 also functions as a powerful genome engi...

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Bibliographic Details
Published in:Nature
Main Authors: Sternberg, Samuel H., LaFrance, Benjamin, Kaplan, Matias, Doudna, Jennifer A.
Format: Artigo
Language:Inglês
Published: 2015
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4859810/
https://ncbi.nlm.nih.gov/pubmed/26524520
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nature15544
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