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A method of predicting the in vitro fibril formation propensity of Aβ40 mutants based on their inclusion body levels in E. coli

Overexpression of recombinant proteins in bacteria may lead to their aggregation and deposition in inclusion bodies. Since the conformational properties of proteins in inclusion bodies exhibit many of the characteristics typical of amyloid fibrils. Based on these findings, we hypothesize that the ra...

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Dettagli Bibliografici
Pubblicato in:Sci Rep
Autori principali: Sanagavarapu, Kalyani, Nüske, Elisabeth, Nasir, Irem, Meisl, Georg, Immink, Jasper N., Sormanni, Pietro, Vendruscolo, Michele, Knowles, Tuomas P. J., Malmendal, Anders, Cabaleiro-Lago, Celia, Linse, Sara
Natura: Artigo
Lingua:Inglês
Pubblicazione: Nature Publishing Group UK 2019
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6403284/
https://ncbi.nlm.nih.gov/pubmed/30842594
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-39216-z
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