Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method

Hepatocytes are parenchymal cells of the liver and engage multiple metabolic functions, including synthesis and secretion of proteins essential for systemic energy homeostasis. Primary hepatocytes isolated from the murine liver constitute a valuable biological tool to understand the functional prope...

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Publicado en:J Vis Exp
Autores principales: Salem, Esam S.B., Murakami, Kazutoshi, Takahashi, Toshimasa, Bernhard, Elise, Borra, Vishnupriya, Bethi, Mridula, Nakamura, Takahisa
Formato: Artigo
Lenguaje:Inglês
Publicado: MyJove Corporation 2018
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC6235588/
https://ncbi.nlm.nih.gov/pubmed/30417869
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/58323
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