Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method

Hepatocytes are parenchymal cells of the liver and engage multiple metabolic functions, including synthesis and secretion of proteins essential for systemic energy homeostasis. Primary hepatocytes isolated from the murine liver constitute a valuable biological tool to understand the functional prope...

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Dettagli Bibliografici
Pubblicato in:J Vis Exp
Autori principali: Salem, Esam S.B., Murakami, Kazutoshi, Takahashi, Toshimasa, Bernhard, Elise, Borra, Vishnupriya, Bethi, Mridula, Nakamura, Takahisa
Natura: Artigo
Lingua:Inglês
Pubblicazione: MyJove Corporation 2018
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Retraction
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6235588/
https://ncbi.nlm.nih.gov/pubmed/30417869
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/58323
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