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Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method

Hepatocytes are parenchymal cells of the liver and engage multiple metabolic functions, including synthesis and secretion of proteins essential for systemic energy homeostasis. Primary hepatocytes isolated from the murine liver constitute a valuable biological tool to understand the functional prope...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:J Vis Exp
Egile Nagusiak: Salem, Esam S.B., Murakami, Kazutoshi, Takahashi, Toshimasa, Bernhard, Elise, Borra, Vishnupriya, Bethi, Mridula, Nakamura, Takahisa
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: MyJove Corporation 2018
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC6235588/
https://ncbi.nlm.nih.gov/pubmed/30417869
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/58323
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