Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method

Hepatocytes are parenchymal cells of the liver and engage multiple metabolic functions, including synthesis and secretion of proteins essential for systemic energy homeostasis. Primary hepatocytes isolated from the murine liver constitute a valuable biological tool to understand the functional prope...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Salem, Esam S.B., Murakami, Kazutoshi, Takahashi, Toshimasa, Bernhard, Elise, Borra, Vishnupriya, Bethi, Mridula, Nakamura, Takahisa
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2018
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Retraction
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6235588/
https://ncbi.nlm.nih.gov/pubmed/30417869
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/58323
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