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Filter Retardation Assay for Detecting and Quantifying Polyglutamine Aggregates Using Caenorhabditis elegans Lysates

Protein aggregation is a hallmark of several neurodegenerative diseases and is associated with impaired protein homeostasis. This imbalance is caused by the loss of the protein’s native conformation, which ultimately results in its aggregation or abnormal localization within the cell. Using a C. ele...

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Detalhes bibliográficos
Publicado no:Bio Protoc
Main Authors: Sin, Olga, Mata-Cabana, Alejandro, Seinstra, Renée I., Nollen, Ellen A. A.
Formato: Artigo
Idioma:Inglês
Publicado em: Bio-Protocol 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6235101/
https://ncbi.nlm.nih.gov/pubmed/30450365
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3042
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