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Filter Retardation Assay for Detecting and Quantifying Polyglutamine Aggregates Using Caenorhabditis elegans Lysates

Protein aggregation is a hallmark of several neurodegenerative diseases and is associated with impaired protein homeostasis. This imbalance is caused by the loss of the protein’s native conformation, which ultimately results in its aggregation or abnormal localization within the cell. Using a C. ele...

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Bibliographische Detailangaben
Veröffentlicht in:Bio Protoc
Hauptverfasser: Sin, Olga, Mata-Cabana, Alejandro, Seinstra, Renée I., Nollen, Ellen A. A.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Bio-Protocol 2018
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6235101/
https://ncbi.nlm.nih.gov/pubmed/30450365
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3042
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