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Conformational changes in Arp2/3 complex induced by ATP, WASp-VCA, and actin filaments

We used fluorescence spectroscopy and EM to determine how binding of ATP, nucleation-promoting factors, actin monomers, and actin filaments changes the conformation of Arp2/3 complex during the process that nucleates an actin filament branch. We mutated subunits of Schizosaccharomyces pombe Arp2/3 c...

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Detalles Bibliográficos
Publicado en:Proc Natl Acad Sci U S A
Main Authors: Espinoza-Sanchez, Sofia, Metskas, Lauren Ann, Chou, Steven Z., Rhoades, Elizabeth, Pollard, Thomas D.
Formato: Artigo
Idioma:Inglês
Publicado: National Academy of Sciences 2018
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC6140485/
https://ncbi.nlm.nih.gov/pubmed/30150414
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1717594115
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