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Mechanism of actin polymerization revealed by cryo-EM structures of actin filaments with three different bound nucleotides

We used cryo-electron microscopy (cryo-EM) to reconstruct actin filaments with bound AMPPNP (β,γ-imidoadenosine 5′-triphosphate, an ATP analog, resolution 3.1 Å), ADP-P(i) (ADP with inorganic phosphate, resolution 3.1 Å), or ADP (resolution 3.6 Å). Subunits in the three filaments have similar backbo...

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Publié dans:Proc Natl Acad Sci U S A
Auteurs principaux: Chou, Steven Z., Pollard, Thomas D.
Format: Artigo
Langue:Inglês
Publié: National Academy of Sciences 2019
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Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6410863/
https://ncbi.nlm.nih.gov/pubmed/30760599
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1807028115
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