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Two-step mechanism and step-arrest mutants of Runella slithyformis NAD(+)-dependent tRNA 2′-phosphotransferase Tpt1
Tpt1 catalyzes the transfer of an internal 2′-monophosphate moiety (2′-PO(4)) from a “branched” 2′-PO(4) RNA splice junction to NAD(+) to form a “clean” 2′-OH, 3′-5′ phosphodiester junction, ADP-ribose 1″-2″ cyclic phosphate, and nicotinamide. First discovered as an essential component of the Saccha...
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| Udgivet i: | RNA |
|---|---|
| Main Authors: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Cold Spring Harbor Laboratory Press
2018
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6097658/ https://ncbi.nlm.nih.gov/pubmed/29884622 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.067165.118 |
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