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Two-step mechanism and step-arrest mutants of Runella slithyformis NAD(+)-dependent tRNA 2′-phosphotransferase Tpt1

Tpt1 catalyzes the transfer of an internal 2′-monophosphate moiety (2′-PO(4)) from a “branched” 2′-PO(4) RNA splice junction to NAD(+) to form a “clean” 2′-OH, 3′-5′ phosphodiester junction, ADP-ribose 1″-2″ cyclic phosphate, and nicotinamide. First discovered as an essential component of the Saccha...

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Библиографические подробности
Опубликовано в: :RNA
Главные авторы: Munir, Annum, Abdullahu, Leonora, Damha, Masad J., Shuman, Stewart
Формат: Artigo
Язык:Inglês
Опубликовано: Cold Spring Harbor Laboratory Press 2018
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC6097658/
https://ncbi.nlm.nih.gov/pubmed/29884622
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.067165.118
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