An expanded toolkit for gene tagging based on MiMIC and scarless CRISPR tagging in Drosophila

We generated two new genetic tools to efficiently tag genes in Drosophila. The first, Double Header (DH) utilizes intronic MiMIC/CRIMIC insertions to generate artificial exons for GFP mediated protein trapping or T2A-GAL4 gene trapping in vivo based on Cre recombinase to avoid embryo injections. DH...

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Detalles Bibliográficos
Publicado en:eLife
Autores principales: Li-Kroeger, David, Kanca, Oguz, Lee, Pei-Tseng, Cowan, Sierra, Lee, Michael T, Jaiswal, Manish, Salazar, Jose Luis, He, Yuchun, Zuo, Zhongyuan, Bellen, Hugo J
Formato: Artigo
Lenguaje:Inglês
Publicado: eLife Sciences Publications, Ltd 2018
Materias:
Genetics and Genomics
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC6095692/
https://ncbi.nlm.nih.gov/pubmed/30091705
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.38709
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