An expanded toolkit for gene tagging based on MiMIC and scarless CRISPR tagging in Drosophila

We generated two new genetic tools to efficiently tag genes in Drosophila. The first, Double Header (DH) utilizes intronic MiMIC/CRIMIC insertions to generate artificial exons for GFP mediated protein trapping or T2A-GAL4 gene trapping in vivo based on Cre recombinase to avoid embryo injections. DH...

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Bibliografski detalji
Izdano u:eLife
Glavni autori: Li-Kroeger, David, Kanca, Oguz, Lee, Pei-Tseng, Cowan, Sierra, Lee, Michael T, Jaiswal, Manish, Salazar, Jose Luis, He, Yuchun, Zuo, Zhongyuan, Bellen, Hugo J
Format: Artigo
Jezik:Inglês
Izdano: eLife Sciences Publications, Ltd 2018
Teme:
Genetics and Genomics
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6095692/
https://ncbi.nlm.nih.gov/pubmed/30091705
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.38709
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