An expanded toolkit for gene tagging based on MiMIC and scarless CRISPR tagging in Drosophila

We generated two new genetic tools to efficiently tag genes in Drosophila. The first, Double Header (DH) utilizes intronic MiMIC/CRIMIC insertions to generate artificial exons for GFP mediated protein trapping or T2A-GAL4 gene trapping in vivo based on Cre recombinase to avoid embryo injections. DH...

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Detalhes bibliográficos
Publicado no:eLife
Main Authors: Li-Kroeger, David, Kanca, Oguz, Lee, Pei-Tseng, Cowan, Sierra, Lee, Michael T, Jaiswal, Manish, Salazar, Jose Luis, He, Yuchun, Zuo, Zhongyuan, Bellen, Hugo J
Formato: Artigo
Idioma:Inglês
Publicado em: eLife Sciences Publications, Ltd 2018
Assuntos:
Genetics and Genomics
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6095692/
https://ncbi.nlm.nih.gov/pubmed/30091705
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.38709
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