A library of MiMICs allows tagging of genes and reversible, spatial and temporal knockdown of proteins in Drosophila

Here, we document a collection of ∼7434 MiMIC (Minos Mediated Integration Cassette) insertions of which 2854 are inserted in coding introns. They allowed us to create a library of 400 GFP-tagged genes. We show that 72% of internally tagged proteins are functional, and that more than 90% can be image...

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Detalhes bibliográficos
Publicado no:eLife
Main Authors: Nagarkar-Jaiswal, Sonal, Lee, Pei-Tseng, Campbell, Megan E, Chen, Kuchuan, Anguiano-Zarate, Stephanie, Cantu Gutierrez, Manuel, Busby, Theodore, Lin, Wen-Wen, He, Yuchun, Schulze, Karen L, Booth, Benjamin W, Evans-Holm, Martha, Venken, Koen JT, Levis, Robert W, Spradling, Allan C, Hoskins, Roger A, Bellen, Hugo J
Formato: Artigo
Idioma:Inglês
Publicado em: eLife Sciences Publications, Ltd 2015
Assuntos:
Cell Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4379497/
https://ncbi.nlm.nih.gov/pubmed/25824290
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.05338
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